As Jessilina her disguise form, she wears an orange sleeveless dress that resembles an apron with yellow frills in each sides along with two pockets in the sides of her puffy dress and brown cowboy boots. This disguise is usually her coordinator form with her beloved Dustox.Underneath, she wears a white long sleeve button shirt with a dark blue laced ribbon on the collar. In the events of Pokemon: Black & White , after she was promoted to Advanced Agent, Jessie's Team Rocket uniform is now charcoal gray before it changes back to the original white after Battling with the Love of Bug-Types! In an attempt to hide the fact that she was from Team Rocket, She wears a dark purple trenchcoat, a matching sunglasses and a fedora hat to conceal herself.Her brown belt on her white pants is finally exposed while it was concealed under her previous jacket. during shopping, Jessie wears a light purple elaborate modern sleeveless gown with frill designs and purple heels.She also wears a summer hat on this occassion Jessie is the short tempered one of the Team Rocket trio, having a very tough streak personality of being vain and very prone to anger.However, the trenchcoat is too long, so usually she ends up wearing it with James being the one walking and her being the one on top, sitting on James's shoulders.In the XY series, her disguise have her wearing a long and pale blue sleeved v-neck jacket with a purple belt tucked on her jacket, a white long sleeved button shirt underneath, a white ribbon on the collar, white pants and matching purple heels.Her hair is tied in a large bun with a sapphire shaped jewel with gold outlines at the centre of her head.Later when she registers for the Pokemon Showcase throughout the series and her standard attire, she wears a very short sleeved and dark magenta short dress, a red belt on her waist and brown flats.
When they encounter villains opposed to their personal or organizational goals, the three are usually more than willing to sabotage the evil team, even to the point of assisting Ash and his allies when they finally recognize the threat.Though they are dedicated, the trio are not entirely loyal to Giovanni, and instead treat the assignments he gives them as means to their own ends.Jessie functions as their confident leader, who allows them the freedom to have side jobs and hobbies and doesn't flinch when lying to their boss' face.But in A Tail with a Twist when a wild Seviper manages to bite her hair, it was ripped off and her hair becomes medium length.All these hairstyles made Jessie furious when each of the Pokémon had accidentally cut her long hair.
As revealed in Training Daze, James is Jessie's partner.